Product NameProto-oncogene tyrosine-protein kinase receptor Ret (RET), Chicken Polyclonal Antibody
Product DescriptiongoogleChicken anti-Proto-oncogene tyrosine-protein kinase receptor Ret (RET) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Alternative NamesCadherin family member 12; Ret;
Application(s)ICC, WB
Antibody HostChicken
Antibody TypePolyclonal
SpecificityHuman RET
Species ReactivityHuman
Immunogen DescriptionRecombinant His-tagged extracellular fragment of human RET protein produced using CHO cell line. The extracellular fragment of hRET was expressed and secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant.
Application DetailsWB, ICC. A dilution of 1:5 000 to 1:10 000 is recommended for Western blot and 1:250 to 1:500 for immunocytochemistry. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetProto-oncogene tyrosine-protein kinase receptor Ret (RET)
SpecificityHuman RET
Target Host SpeciesHuman
Species ReactivityHuman
Antibody HostChicken
Antibody TypePolyclonal
Antibody IsotypeIgY
ConjugateUnconjugated
Immunogen DescriptionRecombinant His-tagged extracellular fragment of human RET protein produced using CHO cell line. The extracellular fragment of hRET was expressed and secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant.
Purity DescriptionAffinity purified
FormatLiquid: Concentrated ammonium sulphate in PBS pH 7.4
Reconstitution InstructionsStore antibody stock solution at +4°C upon receipt. DO NOT FREEZE. This product is an (NH4)2SO4 precipitate. To use, mix well by pipetting or vortexing gently prior use, then withdraw the desired amount of antibody stock solution and dilute into your antibody dilution buffer and use normally.Alternatively, material can be centrifuged at 10K x g for 5 minutes and the liquid sulfate solution carefully removed. Then precipitated antibody can be resuspended in 0.1M P, 250mM NaCL, pH 7.4 solution. The solution should then be divided into working aliquots and stored at -80°C until used. Prevent multiple freeze thaw cycles of aliquots for best product life.
Storage InstructionsStore at 2-8°C upon receipt; DO NOT FREEZE. As product is (NH4)2SO4 (ammonium sulfate) precipitate, mix well by pipetting or vortexing prior to use. See reconstitution instructions for more information.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundThe RET proto-oncogene is a receptor tyrosine kinase for members of the glial cell line-derived neurotrophic factor family of extracellular signalling molecules
Immunofluorescence detection of human RET expressed in U2OS cells. RET was visualized using Chicken polyclonal antibody to human RET (dilution 1:500) and Goat anti-chicken IgY - H&L DyLight 550 (dilution 1:200) as secondary antibody. For nuclear staining DAPI was used. Composite picture was generated using pseudocolors green for RET specific signal and blue for nuclei.
Western-Blot detection of human RET expressed in CHO cells. Line 1. hRET-containing CHOEBNALT85 cell culture supernatant. Line 2. Negative control - CHOEBNALT85 cell culture supernatant. 5 uL of supernatant were loaded per line. Primary antibody dilution 1:5000 was used. Rabbit polyclonal to chicken IgY was used as secondary antibody.