Immunohistochemical staining of Neurotrophin 4/5 (NT4/5) in rat cortex using rabbit polyclonal to recombinant human NT4, catalogue number R-102-500
Immunofluorescence analysis of NT4 expression in human DU145 prostate cancer cells. Fixed (4% formaldehyde), permeabilized, and blocked (10% normal horse serum, 0.1% Triton X100) DU145 cells were incubated with NT4 rabbit antibody antibody R-102-500 (10 µg/mL, green) for 1 hour. Primary antibody binding was visualized with a secondary donkey anti-rabbit-CF488A antibody (4 µg/mL, 1 hour incubation). Control cells were incubated with normal rabbit IgG (10 µg/mL) as primary antibody. Cell nuclei were stained with Hoechst dye (blue). Magnification: 100x.