Immunohistochemical staining of Vanilloid Receptor Like Protein (VRL-1) in rat dorsal horn using rabbit polyclonal antibody to rat VRL-1, catalogue number R-078-50.
Western blot of VRL-1 in PC12 cell lysate (RIPA, 80 µg/lane). R-078-50 detects glycosylated VRL-1 monomer at around 90-95 kDa. VRL-1 dimer is also detected by this antibody in this cell lysate preparation. SDS-PAGE: denatured and non- reduced; Transfer: Tris-Glycine buffer; Membrane: nitrocellulose (0.45 µm); Blocking: 5% skim milk in TBST, 2 hours at RT; Primary antibody: overnight at 4°C (2 µg/mL); Secondary antibody: anti-rabbit-HRP (1/6000), 1 hour at RT; Detection: Chemiluminiscence
Western blot analysis of VRL-1 expression in rat cell lysate and tissue homogenates (RIPA extracts). One prominent band at ~90 kDa is observed corresponding to the expected molecular weight of VRL-1 protein. SDS-PAGE: denatured and reduced; Transfer: Towbin's buffer (20% MeOH, no SDS); Membrane: PVDF (0.45 um); Blocking: 5% skim milk in TBST, 1 hour at RT; Primary antibody: overnight at 2-8°C (1 µg/mL); Secondary antibody: anti-rabbit-HRP (1/10,000), 1 hours at RT; Detection: Chemiluminiscence.