Product NameVanilloid receptor-like protein 1 (VRL-1), Rabbit Polyclonal Antibody
Product DescriptiongoogleRabbit anti-Vanilloid receptor-like protein 1 (VRL-1) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Alternative Namesosm-9-like TRP channel 2; OTRPC2
Application(s)IHC-Frozen, WB
Antibody HostRabbit
Antibody TypePolyclonal
SpecificityImmunohistochemical analysis in rat dorsal root ganglia and spinal cord indicates a high level of specificity for this antiserum. Specificity was also shown by Western blot. This antibody is known to react with rat and human VRL-1.
Species ReactivityHuman, Rat
Immunogen DescriptionA synthetic peptide (C-KNSASEEDHLPLQVLQSP) as part of rat VRL-1 protein (aa: 744-761) conjugated to KLH has been used as the immunogen.
Product DescriptionRabbit anti-Vanilloid receptor-like protein 1 (VRL-1) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Application(s)IHC-Frozen, WB
Application DetailsIHC, Immunofluorescence, Western blot and Immunoblot. Recommended to be used at a concentration of 0.5-1 µg for these applications. This antiserum works superbly for immunohistochemistry on free-floating or paraffin embedded sections. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetVanilloid receptor-like protein 1 (VRL-1)
SpecificityImmunohistochemical analysis in rat dorsal root ganglia and spinal cord indicates a high level of specificity for this antiserum. Specificity was also shown by Western blot. This antibody is known to react with rat and human VRL-1.
Target Host SpeciesRat
Species ReactivityHuman, Rat
Antibody HostRabbit
Antibody TypePolyclonal
Antibody IsotypeIgG
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide (C-KNSASEEDHLPLQVLQSP) as part of rat VRL-1 protein (aa: 744-761) conjugated to KLH has been used as the immunogen.
Purity DescriptionAffinity Purified.
FormatLyophilized from PBS, pH 7.4, containing 0.02% sodium azide as preservative.
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Immunohistochemical staining of Vanilloid Receptor Like Protein (VRL-1) in rat trigeminal ganglion using rabbit polyclonal antibody to rat VRL-1, catalogue number R-078-50.
Immunohistochemical staining of Vanilloid Receptor Like Protein (VRL-1) in rat dorsal horn using rabbit polyclonal antibody to rat VRL-1, catalogue number R-078-50.
Western blot of VRL-1 in PC12 cell lysate (RIPA, 80 µg/lane). R-078-50 detects glycosylated VRL-1 monomer at around 90-95 kDa. VRL-1 dimer is also detected by this antibody in this cell lysate preparation. SDS-PAGE: denatured and non- reduced; Transfer: Tris-Glycine buffer; Membrane: nitrocellulose (0.45 µm); Blocking: 5% skim milk in TBST, 2 hours at RT; Primary antibody: overnight at 4°C (2 µg/mL); Secondary antibody: anti-rabbit-HRP (1/6000), 1 hour at RT; Detection: Chemiluminiscence
Western blot analysis of VRL-1 expression in rat cell lysate and tissue homogenates (RIPA extracts). One prominent band at ~90 kDa is observed corresponding to the expected molecular weight of VRL-1 protein. SDS-PAGE: denatured and reduced; Transfer: Towbin's buffer (20% MeOH, no SDS); Membrane: PVDF (0.45 um); Blocking: 5% skim milk in TBST, 1 hour at RT; Primary antibody: overnight at 2-8°C (1 µg/mL); Secondary antibody: anti-rabbit-HRP (1/10,000), 1 hours at RT; Detection: Chemiluminiscence.
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