Product DescriptionRabbit anti-Activity-regulated gene 3.1 protein homolog (Arg3.1) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Application DetailsIHC. A concentration of of 2-4 µg/mL is recommended for this application. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetActivity-regulated gene 3.1 protein homolog (Arg3.1)
SpecificitySpecific for Arg3.1. Rat and Mouse. Other species not yet tested.
Target Host SpeciesHuman
Species ReactivityHuman, Mouse, Rat
Immunogen DescriptionA synthetic peptide from the C terminal of human Arg3.1 protein (ARC protein) conjugated to Blue Carrier Protein. The sequence is homologous with mouse and rat form of Arg3.1.
Purity DescriptionProtein G purified IgG
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 500 µL sterile-filtered 1X PBS, pH 7.2-7.6. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesARC; activity regulated cytoskeletal-associated protein; Activity-regulated gene 3.1 protein homolog; Arg3.1; RCA/ARG3.1; ARC;
Scientific BackgroundArc (also termed activity-regulated cytoskeleton-associated protein or Arg3.1), is an effector immediate early gene whose upregulation has been demonstrated during events of synaptic plasticity. Arg3.1 expression is detectable in neuronal cell bodies and dendrites in the brain regions including striatum and cortex hippocampus, hypothalamus, amygdala.
General ReferencesKajiya K, et al. J. Neurosci. 21:6018-6025(2001). Young J.M, et al. Genome Biol. 4:RESEARCH71.1-RESEARCH71.13(2003). Zhang X., Firestein S. Nat. Neurosci. 5:124-133(2002). Young J.M, et al. Hum. Mol. Genet. 11:535-546(2002). Kelly MP, et al. Neuroscience. 110(4):617-26 (2002).