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Autophagy-related protein 12 (ATG12), Rabbit Polyclonal Antibody

As low as US$317.00
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Catalog Number
R-137
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    Product Info

  • Product Name Autophagy-related protein 12 (ATG12), Rabbit Polyclonal Antibody
  • Product Description google Rabbit anti-Autophagy-related protein 12 (ATG12) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
  • Alternative Names Autophagy-related protein 12; APG12-like; ATG12; APG12; APG12L
  • Application(s) IHC-Frozen, WB
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Specificity IHC and WB confirmed the specificity for ATG12. Human, rat
  • Species Reactivity Human, Rat
  • Immunogen Description A synthetic peptide corresponding to the N-terminal of human ATG12 protein has been used as the immunogen. No immunogenic carrier protein was conjugated to the immunogen. Instead, Adjukine B (see the Adjuvants in biosensis' product list) has been used to orchestrate/boost the immune response.
  • Conjugate Unconjugated
  • Purity Description Protein G purified IgG
  • Regulatory Status For research use only.

    Specifications

  • Product Description Rabbit anti-Autophagy-related protein 12 (ATG12) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
  • Application(s) IHC-Frozen, WB
  • Application Details IHC, immunofluorescence, WB (1-5 µg/mL working concentration), Flow Cytometry (2μg/10^6 cells). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Autophagy-related protein 12 (ATG12)
  • Specificity IHC and WB confirmed the specificity for ATG12. Human, rat
  • Target Host Species Human
  • Species Reactivity Human, Rat
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Antibody Isotype IgG
  • Conjugate Unconjugated
  • Immunogen Description A synthetic peptide corresponding to the N-terminal of human ATG12 protein has been used as the immunogen. No immunogenic carrier protein was conjugated to the immunogen. Instead, Adjukine B (see the Adjuvants in biosensis' product list) has been used to orchestrate/boost the immune response.
  • Purity Description Protein G purified IgG
  • Format Lyophilized
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 500 µL sterile-filtered 1X PBS, pH 7.2-7.6. Centrifuge to remove any insoluble material.
  • Storage Instructions After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names Autophagy-related protein 12; APG12-like; ATG12; APG12; APG12L
  • Uniprot Number O94817
  • Uniprot Number/Name O94817 (ATG_HUMAN)
  • Scientific Background FUNCTION: Required for autophagy. SUBUNIT: Conjugated to ATG5. SUBCELLULAR LOCATION: Cytoplasm. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: Ubiquitous. SIMILARITY: Belongs to the ATG12 family.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Fixing and Permeabilization of cells: Absolute methanol (10 minutes in ice) and 0.1% Tween-20 in PBS, Blocking: 15% Horse serum, Primary antibody: Rabbit Polyclonal antibody to ATG12 (cat # R-137-500, 2μg per ~10^6 cells) for 30 minutes at room temperature, Secondary antibody: Donkey anti-rabbit PE labeled secondary antibody (1:100 fold dilution) with incubation for 20 minutes in dark at room temperature. Normal Rabbit Polyclonal IgG was used as negative control under same conditions (black dashed). Flow cytometry data and results were generated using Orflo MoxiflowTM instrument and protocols. The data demonstrates specific staining of ATG12 expressed in human neuroblastoma SH-SY5Y cell line using cat # R-137-500.