Autophagy protein 5 (ATG5), Rabbit Polyclonal Antibody

Product Info

Product Name Autophagy protein 5 (ATG5), Rabbit Polyclonal Antibody
Product Description google Rabbit anti-Autophagy protein 5 (ATG5) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Alternative Names Autophagy protein 5; APG5-like; APG 5; APG5; Apoptosis-specific protein; ATG5; APG5L; ASP
Application(s) IHC-Frozen, WB
Antibody Host Rabbit
Antibody Type Polyclonal
Specificity IHC and wb confirmed the specificity for ATG5. Human, not yet tested in other species.
Species Reactivity Human
Immunogen Description A synthetic peptide corresponding to the C-terminal of human ATG-5L. No immunogenic carrier protein was conjugated to the immunogen. Instead, Adjukine B (see the Adjuvants in biosensis' product list) has been used to orchestrate/boost the immune response.
Conjugate Unconjugated
Purity Description Protein G purified IgG
Regulatory Status For research use only.

Specifications

Product Description Rabbit anti-Autophagy protein 5 (ATG5) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Application(s) IHC-Frozen, WB
Application Details Flow Cytometry (2 μg/10^6 cells), IHC, immunofluorescence, WB. Use a dilution of 1:200 to 1:1000 for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target Autophagy protein 5 (ATG5)
Specificity IHC and wb confirmed the specificity for ATG5. Human, not yet tested in other species.
Target Host Species Human
Species Reactivity Human
Antibody Host Rabbit
Antibody Type Polyclonal
Antibody Isotype IgG
Conjugate Unconjugated
Immunogen Description A synthetic peptide corresponding to the C-terminal of human ATG-5L. No immunogenic carrier protein was conjugated to the immunogen. Instead, Adjukine B (see the Adjuvants in biosensis' product list) has been used to orchestrate/boost the immune response.
Purity Description Protein G purified IgG
Format Lyophilized
Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 500 µL sterile-filtered 1X PBS, pH 7.2-7.6. Centrifuge to remove any insoluble material.
Storage Instructions After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names Autophagy protein 5; APG5-like; APG 5; APG5; Apoptosis-specific protein; ATG5; APG5L; ASP
Uniprot Number Q9H1Y0
Uniprot Number/Name Q9H1Y0 (ATG5_HUMAN)
Scientific Background FUNCTION: Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. FUNCTION: May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. SUBCELLULAR LOCATION: Cytoplasm. Colocalizes with nonmuscle actin. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells. INDUCTION: By apoptotic stimuli. PTM: Conjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane. SIMILARITY: Belongs to the ATG5 family.
Shipping Temperature 25°C (ambient)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

Fixing and Permeabilization of cells: Absolute methanol (10 minutes in ice) and 0.1% Tween-20 in PBS, Blocking: 15% Horse serum, Primary antibody: Rabbit Polyclonal antibody to ATG5 (cat # R-138-500, 2 ug per ~10^6 cells) for 30 minutes at room temperature, Secondary antibody: Donkey anti-rabbit PE labeled secondary antibody (1:100 fold dilution) with incubation for 20 minutes in dark at room temperature. Normal Rabbit Polyclonal IgG was used as negative control under same conditions (black dashed). Flow cytometry data and results were generated using Orflo MoxiflowTM instrument and protocols. The data demonstrates specific staining of ATG5 expressed in human neuroblastoma SH-SY5Y cell line using cat # R-138-500.

Citations & References

Specific References Villanueva-Paz M. et al. (2016) Amitriptyline induces mitophagy that precedes apoptosis in human HepG2 cells. Genes Cancer. 2016;7(7-8):260-277
Garrido-Maraver J. et al (2012) Screening of effective pharmacological treatments for MELAS syndrome using yeasts, fibroblasts and cybrids models of the disease Br J Pharmacol. 2012 Jul 2.
De la Mata M. et al (2012) Recovery of MERRF fibroblasts and cybrids pathophysiology by Coenzyme Q10 Neurotherapeutics. 2012 Apr;9(2):446-63.
Cotan D. et al. (2011) Secondary coenzyme Q10 deficiency triggers mitochondria degradation by mitophagy in MELAS fibroblasts FASEB J. 2011
General References Mizushima, N et al. (2003) Int J Biochem Cell Biol. 35(5), 553-61
Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-(2005)
Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-4(2005)
Greenberg JT. Dev Cell. 8(6):799-80(2005)