Autophagy-related protein 9A (ATG9A), Rabbit Polyclonal Antibody

Product Info

Product Name Autophagy-related protein 9A (ATG9A), Rabbit Polyclonal Antibody
Product Description google Rabbit anti-Autophagy-related protein 9A (ATG9A) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Alternative Names Autophagy-related protein 9A; APG9-like 1; Autophagy-related protein 9; APG9A; ATG9A; APG9L1
Application(s) IHC-Frozen, WB
Antibody Host Rabbit
Antibody Type Polyclonal
Specificity IHC, WB and ELISA confirmed the specificity for ATG9A (APG9L1) . Human, rat. Other species not yet tested.
Species Reactivity Human, Rat
Immunogen Description A synthetic peptide (CWHRRESDESGE) corresponding to the C-terminal of human APG9A (APG9L1) protein has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from APG9A (APG9L1) protein in mouse, rat, S. cerevisiae, Macaca mulatta (monkey) and Canis familiaris (dog).
Conjugate Unconjugated
Purity Description Whole serum
Regulatory Status For research use only.

Specifications

Product Description Rabbit anti-Autophagy-related protein 9A (ATG9A) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Application(s) IHC-Frozen, WB
Application Details IHC, immunofluorescence, WB. A dilution of 1:100 to 1:3000 dilution is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target Autophagy-related protein 9A (ATG9A)
Specificity IHC, WB and ELISA confirmed the specificity for ATG9A (APG9L1) . Human, rat. Other species not yet tested.
Target Host Species Human
Species Reactivity Human, Rat
Antibody Host Rabbit
Antibody Type Polyclonal
Antibody Isotype Mixed
Conjugate Unconjugated
Immunogen Description A synthetic peptide (CWHRRESDESGE) corresponding to the C-terminal of human APG9A (APG9L1) protein has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from APG9A (APG9L1) protein in mouse, rat, S. cerevisiae, Macaca mulatta (monkey) and Canis familiaris (dog).
Purity Description Whole serum
Format Lyophilized
Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage Instructions After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names Autophagy-related protein 9A; APG9-like 1; Autophagy-related protein 9; APG9A; ATG9A; APG9L1
Uniprot Number Q7Z3C6
Uniprot Number/Name Q7Z3C6 (ATG9A_HUMAN)
Scientific Background FUNCTION: Plays a role in autophagy. SUBCELLULAR LOCATION: Membrane; multi-pass membrane protein (Potential). ALTERNATIVE PRODUCTS: 3 named isoforms produced by alternative splicing. SIMILARITY: Belongs to the ATG9 family. IN YEAST: FUNCTION: Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Recruites ATG23 and ATG8 to the pre-autophagosomal structure. SUBUNIT: Interacts with ATG18, ATG2 and ATG23. SUBCELLULAR LOCATION: Membrane; multi-pass membrane protein. Preautophagosomal structure; preautophagosomal structure membrane; multi-pass membrane protein. Note=Pre-autophagosomal and other perivacuolar punctate structures. The proper trafficking of ATG9 between the pre-autophagosomal structure and the other punctate structures requires ATG2, ATG18, ATG23, the ATG1-ATG13 complex and the phosphatidylinositol 3-kinase complex I. SIMILARITY: Belongs to the ATG9 family.
Shipping Temperature 25°C (ambient)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

Confocal microscopy on mouse lymph node using Rabbit antibody to ATG9A (APG9L1): whole serum (R-160-100).
Western blot (non-reducing) on Jurkat cell lysate using Rabbit antibody to ATG9A (APG9L1): whole serum (R-160-100).

Western blot on Peripheral Blood Mononuclear Cells (PBMC) lysate using Rabbit antibody to ATG9A (APG9L1): whole serum (R-160-100) at a dilution of 1: 100 (ECL).

Citations & References

Specific References Park YE et al (2009) Autophagic degradation of nuclear components in mammalian cells. Autophagy. 2009 Aug;5(6):795-804.
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Lang T, et al. J. Bacteriol. 182:2125-2133(2000).
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Kim H, et al. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
Tucker K.A, et al. J. Biol. Chem. 278:48445-48452(2003).