Application DetailsWestern Blot (0.1-0.5 µg/mL): tested on human and rat cell lines. Other applications not yet tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetEpidermal growth factor receptor (EGFR)
SpecificityThis antibody is specific for EGFR as demonstrated by western blotting.
Target Host SpeciesHuman
Species ReactivityHuman, Rat
Immunogen DescriptionE.coli-derived human EGFR recombinant protein (Position: L25-K346). Human EGFR shares 89% amino acid (aa) sequence identity with mouse EGFR.
Purity DescriptionAffinity purified
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 uL sterile-filtered, ultrapure water to prepare 1 mg/mL solution. Centrifuge to remove any insoluble material.
Storage InstructionsStore lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundEpidermal Growth Factor (EGF) Receptor binds to EGF and other members of the EGF family such as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. At least 4 isoforms are produced by alternative splicing (reference SWISSPROT). The EGF receptor is a single-pass type I membrane protein containing an extracellular receptor domain, a transmembrane region and an intracellular domain with tyrosine kinase function