Application DetailsWestern blotting (1:1,000-1:5,000) and Immunocytochemistry (1:1,000-1:2,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Immunogen DescriptionFull-length recombinant human protein
Purity DescriptionWhole serum
FormatLyophilized, with 5mM sodium azide.
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsStore lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundMay participate in RNA metabolism in the myelinating cell, CNP is the third most abundant protein in central nervous system myelin. Ref: uniprot.org
Left: Mixed neuron-glial cell cultures stained with rabbit anti-CNP antibody (green) and mouse anti-GFAP (red, M-1375-100) by Immunocytochemistry. The CNP antibody stains strongly in oligodendrocytes, whereas GFAP labels the intermediate filament in astrocytes. Blue is DNA staining showing the nuclei of these and numerous other cells, most of which are neurons and which do not express CNP or GFAP. Right: Western Blotting of rat brain tissue homogenate with rabbit anti-CNP antibody (1:5,000). The antibody detects one single band at ~ 48 kDa corresponding to the expected molecular weight of CNP.
Western blot analysis using rabbit anti-CNP antibody (red, 1:5,000). [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, and [6] cow spinal cord. Double bands at 46 and 48 kDa correspond to the two CNP isotypes. The blot was simultaneously probed with mouse anti-calretinin antibody (green, 29 kDa).
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