2',3'-cyclic-nucleotide 3'-phosphodiesterase (CNPase), Rabbit Polyclonal Antibody

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Catalog Number
R-1803

    Product Info

  • Product Name 2',3'-cyclic-nucleotide 3'-phosphodiesterase (CNPase), Rabbit Polyclonal Antibody
  • Product Description google
    Rabbit anti-2',3'-cyclic-nucleotide 3'-phosphodiesterase (CNP) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
  • Application(s) ICC, WB
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Specificity Human,reacts with Human, Rat, Mouse.
  • Species Reactivity Human, Mouse, Rat
  • Immunogen Description Full-length recombinant human protein
  • Conjugate Unconjugated
  • Purity Description Whole serum
  • Regulatory Status For research use only.

    Specifications

  • Product Description
    Rabbit anti-2',3'-cyclic-nucleotide 3'-phosphodiesterase (CNP) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
  • Application(s) ICC, WB
  • Application Details Western blotting (1:1,000-1:5,000) and Immunocytochemistry (1:1,000-1:2,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target 2',3'-cyclic-nucleotide 3'-phosphodiesterase (CNP)
  • Specificity Human,reacts with Human, Rat, Mouse.
  • Target Host Species Human
  • Species Reactivity Human, Mouse, Rat
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Conjugate Unconjugated
  • Immunogen Description Full-length recombinant human protein
  • Purity Description Whole serum
  • Format Lyophilized with sodium azide.
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
  • Storage Instructions Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Uniprot Number P09543
  • Uniprot Number/Name P09543 (CN37_HUMAN)
  • Scientific Background May participate in RNA metabolism in the myelinating cell, CNP is the third most abundant protein in central nervous system myelin. Ref: uniprot.org
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Left: Mixed neuron-glial cell cultures stained with rabbit anti-CNP antibody (green) and mouse anti-GFAP (red, M-1375-100) by Immunocytochemistry. The CNP antibody stains strongly in oligodendrocytes, whereas GFAP labels the intermediate filament in astrocytes. Blue is DNA staining showing the nuclei of these and numerous other cells, most of which are neurons and which do not express CNP or GFAP. Right: Western Blotting of rat brain tissue homogenate with rabbit anti-CNP antibody (1:5,000). The antibody detects one single band at ~ 48 kDa corresponding to the expected molecular weight of CNP.

  • Western blot analysis using rabbit anti-CNP antibody (red, 1:5,000). [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, and [6] cow spinal cord. Double bands at 46 and 48 kDa correspond to the two CNP isotypes. The blot was simultaneously probed with mouse anti-calretinin antibody (green, 29 kDa).

    Citations & References