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Mesencephalic astrocyte-derived neurotrophic factor (MANF), Rabbit Polyclonal Antibody
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Catalog Number
R-1675
- Product Name Mesencephalic astrocyte-derived neurotrophic factor (MANF), Rabbit Polyclonal Antibody
- Product Description Rabbit anti-Mesencephalic astrocyte-derived neurotrophic factor (MANF) Polyclonal Antibody (Unconjugated), suitable for WB.
- Alternative Names ARMET, ARP
- Application(s) WB
- Antibody Host Rabbit
- Antibody Type Polyclonal
- Specificity MANF (Mesencephalic astrocyte-derived neurotrophic factor). No cross-reactivity in MANF-knockout mouse. No cross-reactivity with CDNF in IHC, slightly cross-reacts with purified CDNF in WB. No cross-reactivity with CDNF in IHC, slightly cross-reacts with purified CDNF in WB.
- Species Reactivity Human
- Immunogen Description Recombinant human MANF protein produced using CHO-based cell line. Immunogen is purified from cell culture supernatant.
- Conjugate Unconjugated
- Purity Description Affinity purified.
- Regulatory Status For research use only.
Product Info
- Product Description Rabbit anti-Mesencephalic astrocyte-derived neurotrophic factor (MANF) Polyclonal Antibody (Unconjugated), suitable for WB.
- Application(s) WB
- Application Details Western blot (WB) at a suggested dilution of 1:2,000 - 1:6,000. Immunohistochemistry (IHC) and Immunofluorescence (IF). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
- Target Mesencephalic astrocyte-derived neurotrophic factor (MANF)
- Specificity MANF (Mesencephalic astrocyte-derived neurotrophic factor). No cross-reactivity in MANF-knockout mouse. No cross-reactivity with CDNF in IHC, slightly cross-reacts with purified CDNF in WB. No cross-reactivity with CDNF in IHC, slightly cross-reacts with purified CDNF in WB.
- Target Host Species Human
- Species Reactivity Human
- Antibody Host Rabbit
- Antibody Type Polyclonal
- Antibody Isotype IgG
- Conjugate Unconjugated
- Immunogen Description Recombinant human MANF protein produced using CHO-based cell line. Immunogen is purified from cell culture supernatant.
- Purity Description Affinity purified.
- Format Concentrated ammonium sulfate in PBS pH 7.4 containing no preservatives.
-
Reconstitution Instructions
Store antibody stock solution at +4°C upon receipt. DO NOT FREEZE. This product is an (NH4)2SO4 precipitate. To use, mix well by pipetting or vortexing gently prior use, then withdraw the desired amount of antibody stock solution and dilute into your antibody dilution buffer and use normally.
Alternatively, material can be centrifuged at 10K x g for 5 minutes and the liquid sulfate solution carefully removed. Then precipitated antibody can be resuspended in 0.1M P, 250mM NaCL, pH 7.4 solution. The solution should then be divided into working aliquots and stored at -80°C until used. Prevent multiple freeze thaw cycles of aliquots for best product life. - Storage Instructions Store antibody stock solution at 2-8°C upon receipt. DO NOT FREEZE. This product is an (NH4)2SO4 precipitate. See reconstitution instructions for more information.
- Batch Number Please see item label.
- Expiration Date 12 months after date of receipt (unopened vial).
- Alternative Names ARMET, ARP
- Uniprot Number P55145
- Uniprot Number/Name P55145 (MANF_HUMAN)
- Scientific Background MANF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- induced degeneration of dopamine neurons in rodent models of Parkinson's disease (Lindholm et al., 2008, Voutilainen et al., 2009). When administered after 6-OHDA-lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116161
- Regulatory Status For research use only.
