Left: SH-SY5Y neuroblastoma cells stained with rabbit antibody to Nestin (red, 1:5,000) and chicken antibody to Vimentin (C-1409-50, green, 1:10,000) by Immunocytochemistry. Vimentin is main partner of nestin forming heterodimers, further polymerizing to form intermediate filaments. Co-localization of vimentin and nestin in cells is shown in yellow. Blue: DAPI staining of nuclear DNA. Right: Western blot analysis of nestin expression in SH-SY5Y cell lysate. The antibody (1:2,000) detects one specific band running at ~260 kDa corresponding to full-length nestin protein.