Application DetailsWB. A dilution of 1:500 to 1:1000 is recommended for this application. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetSuperoxide dismutase 1 (SOD1)
SpecificitySpecificity was confirmed by western blot detecting human superoxide dismutase (SOD1). This antiserum is known to react with human superoxide dismutase (SOD1).
Target Host SpeciesHuman
Immunogen DescriptionA synthetic peptide (ESNGPVK VWGSIK) as part of human superoxide dismutase (SOD1) protein (aa: 24-36) conjugated to diphtheria toxoid
Purity DescriptionWhole serum
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesSuperoxide dismutase [Cu-Zn]; SOD1
Scientific BackgroundFUNCTION: Destroys radicals which are normally produced within the cells and which are toxic to biological systems. CATALYTIC ACTIVITY: 2 superoxide + 2 H+ = O2 + H2O2. COFACTOR: Binds 1 copper ion per subunit. COFACTOR: Binds 1 zinc ion per subunit. SUBUNIT: Homodimer. SUBCELLULAR LOCATION: Cytoplasm. DISEASE: Defects in SOD1 are the cause of familial amyotrophic lateral sclerosis (FALS); also called amyotrophic lateral sclerosis 1 (ALS1 or ALS). ALS is a degenerative disorder of motorneurons in the cortex, brainstem and spinal cord. ALS is characterized by muscular weakness and atrophy beginning in the hands and spreading to the forearms and legs. Muscle fasciculations are commonly visible. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. ALS is sometimes referred to as Lou Gehrig disease after the famous American baseball player who was diagnosed with the disorder. FALS, the familial form of ALS, accounts for about 10% of the cases and is transmitted in an autosomal dominant manner. The mean age at onset of FALS is 45 years. MISCELLANEOUS: Zinc binding promotes dimerization. SIMILARITY: Belongs to the Cu-Zn superoxide dismutase family.
Western blot detection of human SOD1 using Rabbit antibody to human superoxide dismutase (SOD1; 24-36): whole serum (R-125-100) at a dilution of 1 in 500. This antibody detects a band of 16KDa in human Jurkat T cell extracts (lane 2). Its specificity for human SOD1 is shown by the lack of reactivity with mouse brain tissue extract (lane 1).
General ReferencesJabusch J.R, et al. Biochemistry 19:2310-2316(1980). Levanon D, et al. EMBO J. 4:77-84(1985). Hallewell R.A, et al. Nucleic Acids Res. 13:2017-2034(1985). Sherman L, et al. Proc. Natl. Acad. Sci. U.S.A. 80:5465-5469(1983). Kajihara J, et al. J. Biochem. 104:851-854(1988). Alexander M.D, et al. Ann. Neurol. 52:680-683(2002). Gellera C, et al. Neuromuscul. Disord. 11:404-410(2001). Murakami T, et al. J. Neurol. Sci. 189:45-47(2001). Penco S, et al. Neurology 53:404-406(1999). Morita M, et al. Neurosci. Lett. 205:79-82(1996). 1Bartlett, et al. J. Neuroscience Methods. 98(1): 63-7(2000)