Left: Analysis of rat brain stem section stained with rabbit anti-laminin (red, 1:1,000), and chicken anti-myelin basic protein (MBP, C-1383-50, green, 1:5,000) by Immunohistochemistry. Blue: DAPI nuclear stain. IHC Method: Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 um, and free-floating sections were stained.The laminin antibody is an excellent marker of basement membranes surrounding blood vessels, while the MBP antibody stains the myelin sheathes around axons. Right: Western blot analysis of laminin expression (green, 1:5,000).  rat brain,  rat spinal cord, and  cow spinal cord. The strong band above the 250 kDa mark corresponds to full-length laminin protein. Smaller proteolytic fragments of laminin are also detected with this antibody.