Application DetailsIHC, WB, ELISA. This antibody works superbly in Immunohistochemistry on frozen or paraffin embedded tissues. Antigen retrieval has been used in testing but may not be necessary. Typical working dilutions for routine immunohistochemistry are 1: 100 to 1: 1000 depending on tissue and detection method. For western blotting a dilution range of 1: 1000 to 1: 4000 is recommended. A dilution of 1: 1000 to 1: 4000 is recommended for ELISA. This antiserum stains the cytoplasm of epithelial cells in the rat and mouse lung and rat and human brain astrocytes. It stains human brain astrocytes in Parkinson's and Alzheimer 's disease and the central core of some Lewy bodies in Parkinson's disease and dementia with Lewy bodies. Other tissues have not yet been tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
SpecificityThis antibody has been shown to be specific for Peroxiredoxin-6 protein. Rat, human and mouse, other species have not yet been tested.
Target Host SpeciesHuman
Species ReactivityHuman, Mouse, Rat
Immunogen DescriptionRat recombinant Peroxiredoxin-6. The sequence is homologous in human and mouse peroxiredoxin-6.
Purity DescriptionWhole serum
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundFUNCTION: Involved in redox regulation of the cell. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. SUBUNIT: Homotetramer. SUBCELLULAR LOCATION: Cytoplasm. Lysosome. Also found in lung secretory organelles. MISCELLANEOUS: The active site is the redox-active Cys-47 oxidized to Cys-SOH. Cys-SOH may rapidly react with a Cys-SH of the other subunit to form an intermolecular disulfide with a concomitant homodimer formation. The enzyme may be subsequently regenerated by reduction of the disulfide by thioredoxin . MISCELLANEOUS: Irreversibly inactivated by overoxidation of Cys-47 (to Cys-SO(3)H) upon oxidative stress. SIMILARITY: Belongs to the ahpC/TSA family. Rehydrin subfamily.
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Immunohistochemical detection of Peroxiredoxin-6 in bronchiole epithelium of rat lung when using Rabbit antibody to Peroxiredoxin-6 (NSGPx): whole serum, catalogue no. R-169-100, at a dilution of 1:300. The tissue is stained light blue with hematoxylin and the bronchiole lining is stained dark brown with DAB. (Image courtesy of JHT Power).
Specific ReferencesWalsh B. et al. (2010) Overexpression of Prdx6 and resistance to peroxide-induced death in Hepa1-6 cells: Prdx suppression increases apoptosis. Redox Rep. 2009;14(6):275-84. Gardiner F. et al. (2010) Induction of Prdx1 and Prdx6 in Liver Cellsby Serum and TPA. Intl J. Biol. Vol. 2, No. 1
General ReferencesKim T.-S, et al. J. Biol. Chem. 272:2542-2550(1997). Choi H.-J, et al. Nat. Struct. Biol. 5:400-406(1998). Rush J, et al. Nat. Biotechnol. 23:94-101(2005). Wagner E, et al. Biochem. J. 366:777-785(2002). Chen J.-W, et al. J. Biol. Chem. 275:28421-28427(2000). Kang S.W, et al. J. Biol. Chem. 273:6303-6311(1998). Gevaert K, et al. Nat. Biotechnol. 21:566-569(2003). Gregory S.G, et al. ature 441:315-321(2006). Nagase T, et al. DNA Res. 2:37-43(1995). Frank S, et al. Oncogene 14:915-921(1997). 1Power J, et al. Am J. Path. 161: 885-894 (2202). 1Power J, et al. Experimental Lung Res. 25: 379-391(1999).