Cellular oncogene fos (c-FOS), Rabbit Polyclonal Antibody

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Catalog Number
R-1751

    Product Info

  • Product Name Cellular oncogene fos (c-FOS), Rabbit Polyclonal Antibody
  • Product Description google
    Rabbit anti-Cellular oncogene fos (c-FOS) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen, ICC, WB.
  • Alternative Names Cellular oncogene fos; G0/G1 switch regulatory protein 7; cFOS
  • Application(s) ICC, IHC-Frozen, WB
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Species Reactivity Human, Mouse, Rat
  • Immunogen Description Full length, E.coli-derived recombinant human c-FOS protein.
  • Conjugate Unconjugated
  • Purity Description Affinity Purified
  • Regulatory Status For research use only.

    Specifications

  • Product Description
    Rabbit anti-Cellular oncogene fos (c-FOS) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen, ICC, WB.
  • Application(s) ICC, IHC-Frozen, WB
  • Application Details Immunocytochemistry (ICC): 1:2,000-1:10,000.

    Immunohistochemistry (IHC): 1:20,000-1:50,000. This antibody has been shown to work on 4% PFA fixed mouse brain sections. Note that non-specific staining has been observed on tissue sections when using this antibody at dilutions of 1:5,000 or lower. Click here for instructions on use of this antibody in IHC on free-floating brain sections.

    Western blotting (WB): 1:1,000-1:2,000. This antibody detects bands between 50-65 kDa, which only appear in stimulated cells.

    Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Cellular oncogene fos (c-FOS)
  • Target Host Species Human
  • Species Reactivity Human, Mouse, Rat
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Antibody Isotype IgG
  • Conjugate Unconjugated
  • Immunogen Description Full length, E.coli-derived recombinant human c-FOS protein.
  • Purity Description Affinity Purified
  • Format Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute with 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
  • Storage Instructions Store lyophilized, unopened vial at 2-8°C or lower. After reconstitution, prepare aliquots and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names Cellular oncogene fos; G0/G1 switch regulatory protein 7; cFOS
  • Uniprot Number P01100
  • Uniprot Number/Name P01100 (FOS_HUMAN)
  • Scientific Background FUNCTION: Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum. SUBUNIT: Heterodimer. Interacts with DSIPI; this interaction inhibits the binding of active AP1 to its target DNA. Interacts with MAFB. SUBCELLULAR LOCATION: Nucleus. INDUCTION: C-fos expression increases upon a variety of stimuli, including growth factors, cytokines, neurotransmitters, polypeptide hormones, stress and cell injury. SIMILARITY: Belongs to the bZIP family. Fos subfamily. SIMILARITY: Contains 1 bZIP domain (Ref: uniprot.org).
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Analysis of c-Fos expression in cultured HeLa cells by Immunocytochemistry (A-B), and in mouse hippocampus (C) or olfactory bulb sections (D) by Immunohistochemistry. HeLa cells were kept in serum-free medium for 36 hours, before cells were stimulated with 20% FBS for 30 minutes (B), while control cells (A) were treated with PBS as a control. The rabbit anti-c-Fos antibody (red, 1:5,000) labels nuclei of stimulated cells, while DAPI (blue) stains all cell nuclei independent of their activity stage. Green: mouse anti-tubulin antibody. Mouse hippocampus (C) and olfactory bulb sections (D) were stained with rabbit anti-c-Fos antibody (red, 1:20,000) and mouse antibody to NF-L (green). The c-FOS antibody stains only nuclei of spontaneously active neurons. NF-L is expressed in axons of neuronal cells. Blue: Cell nuclei stained with DAPI. IHC Method: Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 uM, and free-floating sections were stained with above antibodies.

  • A: Western blot analysis of c-Fos expression in HeLa cells using R-1751-50. HeLa cells were serum-starved for 36 hours (Lane 1). Serum-starved HeLa cells were stimulated with 20% FBS for 2 hours (Lane 2). R-1751-50 recognizes bands in the range of 50-65 kDa, which represent multiple forms of c-Fos. A loading control was performed by stripping and re-probing the membrane with a monoclonal antibody against GAPDH, M-1376-250.
    B: Immunofluorescence staining of HeLa cells with R-1751-50. c-Fos staining (red) only localizes in the nuclei of 20% FBS stimulated cells (bottom panel), but not in un-stimulated cells (top panel). Cells were counter-stained with Chicken polyclonal antibody against vimentin, C-1409-50 (green) and DAPI (blue).
    C: Immunohistochemistry using R-1751-50 on 4% PFA transcardial-perfused mouse brain sections (45 uM thickness). c-FOS immunoreactive cells (dark colour, localized in cell nucleus) were visualized using a standard HRP-DAB (horseradish peroxidase-diaminobenzidine) staining technique. D: Immunohistochemistry using R-1751-50 (red) and Mouse monoclonal anti-NeuN/Fox3 (M-377-100, green) on mouse cortical sections. Neurons positive for c-Fos and Fox3/NeuN appear yellow. The insert shows an enlarged image of staining with R-1751-50. Nuclei were labeled with DAPI (blue).


    A: Western blot analysis of c-Fos expression in rat C6 cells (40 ug lysate loading per lane). C6 cells were serum-starved for 20 hours and then stimulated with 20% FBS for 2 hrs (+). Control cells were left in serum-depleted culture medium (-). M-1752-100 and R-1751-50 detect a strong band at 50 kDa in stimulated cells but not in control cells, representing increased c-FOS expression.
    B:Western blot analysis of c-Fos expression in PC12 cell lysates (Lane 1, 20 µg), mouse brain (Lane 2, 50 µg) and rat spinal cord homogenate (Lane 3, 50 µg). R-1751-50 detects multiple c-Fos isoforms between ~50-65 kDa which are due to post-translational modifications. In mouse brain, R-1751-50 detects a common c-Fos degradation product (~41 kDa) which appears to be lysate and preparation dependent.
    Western Blotting Method: SDS-PAGE: denaturing and reducing, 4-12% Bis-Tris gel; Transfer: Tris-Glycine (Towbin's) buffer with 20% methanol; Membrane: PVDF (0.45 µm); Blocking: 5% skim milk in TBST, 1 hour at RT; Primary antibodies: R-1751-50 (1 µg/mL), M-1752-100 (1 µg/mL), incubation overnight at 4°C; Secondary antibodies: anti-rabbit-HRP (1/6000) or anti-mouse-HRP (1/3000), 1 hour at RT; Detection: Chemiluminiscence.


    Western blot analysis of cell lysates using rabbit antibody to c-FOS (green, 1:5,000), and mouse antibody to GAPDH (M-1376-250, red, 1:5,000, lanes 2-5) used as a loading control. [1] protein standard, [2] HeLa cells grown in FBS free media, [3] HeLa cells stimulated with 20% FBS for 2 hours after being in FBS free media for 36 hours, [4] rat cortical neurons, [5] rat cortical neurons treated with membrane depolarization buffer for 5 hours. Multiple bands at 50-65 kDa in stimulated or treated cell lysates correspond to different isoforms of the c-FOS protein.

    Citations & References

  • Specific References

    Gildawie KR et al. (2023). Effects of Maternal Separation on Effort-based Responding for Sucrose Are Associated with c-Fos Expression in the Nucleus Accumbens Core Neuroscience. [Epub ahead of print] Application: IHC (IF). Species: Rat

    Kim JE et al. (2023). A small-molecule degrader of TET3 as treatment for anorexia nervosa in an animal model Proc Natl Acad Sci USA. [Epub ahead of print] Application: IHC (IF). Species: Mouse.

    Lin King JV (2020). Stinging Sensations: Activation Mechanisms of the Wasabi Receptor, TRPA1. PhD Thesis. Application: IHC (IF). Species: Mouse.

    Lin King JV et al. (2019). A Cell-Penetrating Scorpion Toxin Enables Mode-Specific Modulation of TRPA1 and Pain. Cell. [Epub ahead of print]. Application: IHC. Species: Mouse.

  • General References Vanstraaten et al (1983) Proc. Natl. Acad. Sci. 80: 3183 (Original molecular c-fos sequence paper)

    Minson J. et al. (1994) Brain Res. 646: 44-52 (Early IHC localization paper)