Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. Human Annexin V has a predicted length of 320 residues and a MW of 36 kDa. A concentration of 0.5-1 µg/mL is recommended to detect Annexin V in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; rat; predicted to react with mouse due to sequence homology;
Target Host SpeciesHuman
Species ReactivityHuman, Mouse (Predicted), Rat
Immunogen DescriptionA synthetic peptide corresponding to a region (88-102 aa) from human Annexin V.
Purity DescriptionAffinity purified on antigen column
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundAnnexin V, also known as Annexin A5, belongs to the annexin family of calcium dependent phospholipid binding proteins. Annexin V has roles in cellular signal transduction, inflammation, growth and differentiation. Annexin V has been shown to have a high affinity for phosphatidylserine (PS) and is therefore a potential probe for the detection of apoptosis.