Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1.0-2.0 µg/mL is recommended for WB. Human Apoptosis Inhibitor 5 (isoform 1) has a predicted length of 510 residues and MW of 58 kDa. A concentration of 0.5-1.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetApoptosis Inhibitor 5 (API-5)
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; mouse; rat; rabbit;
Target Host SpeciesHuman
Species ReactivityHuman, Mouse, Rabbit, Rat
Immunogen DescriptionA synthetic peptide (RQIYNPPSGKYSSNL) corresponding to a region (478-492) from the C-terminus of human Apoptosis Inhibitor 5.
Purity DescriptionAffinity purified on antigen column
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundApoptosis Inhibitor 5 is an anti-apoptotic factor that is widely expressed with higher levels in heart, pancreas and placenta. It is also highly expressed in several cancers. At least 4 isoforms are produced by alternative splicing.