Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1.0-2.0 µg/mL is recommended for WB. Human Aquaporin 1 has a predicted length of 269 amino acids and MW of 29 kDa. A concentration of 0.5-1.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; mouse; rat; rabbit;
Target Host SpeciesHuman
Species ReactivityHuman, Mouse, Rabbit, Rat
Immunogen DescriptionA synthetic peptide (KVWTSGQVEEYDLDAD) corresponding to a region (243-258) from human Aquaporin 1.
Purity DescriptionAffinity purified on antigen column
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesAquaporin-CHIP; Water channel protein for red blood cells and kidney proximal tubule; Urine water channel; AQP-1; CHIP28; AQP1;
Scientific BackgroundAquaporin 1 is a multi-pass membrane protein expressed in tissues including renal tubules, kidney, pancreas, heart, lung and erythrocytes. Aquaporin 1 functions as a water-specific channel that provides the plasma membrane with a high permeability to water thereby allowing water to move through the membrane.