Atrial Natriuretic Peptide, Rabbit Polyclonal Antibody

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Catalog Number
R-1500

    Product Info

  • Product Name Atrial Natriuretic Peptide, Rabbit Polyclonal Antibody
  • Product Description google Rabbit anti-Atrial Natriuretic Peptide Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen, IHC-Paraffin-embedded, ICC.
  • Alternative Names Atrial natriuretic factor; ANF; Prepronatriodilatin;
  • Application(s) ICC, IHC-Frozen, IHC-Paraffin-embedded
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Specificity Human; mouse; rat. Highly conserved and expected to interact with feline, canine, porcine, bovine, and equine ANP.
  • Species Reactivity Bovine (Predicted), Cat (Predicted), Dog (Predicted), Horse (Predicted), Human, Mouse, Pig (Predicted), Rat
  • Immunogen Description Synthetic rat Atrial natriuretic peptide (123-150 aa) conjugated to BSA.
  • Conjugate Unconjugated
  • Purity Description Affinity-purified
  • Regulatory Status For research use only.

    Specifications

  • Product Description Rabbit anti-Atrial Natriuretic Peptide Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen, IHC-Paraffin-embedded, ICC.
  • Application(s) ICC, IHC-Frozen, IHC-Paraffin-embedded
  • Application Details A dilution of 5-10 µg/mL is recommended for immunohistochemistry using formalin fixed and paraffin embedded tissues and for 4% paraformaldehyde fixed frozen tissues. A dilution of 5-15 µg/mL is recommended for immunofluorescence. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Atrial Natriuretic Peptide
  • Specificity Human; mouse; rat. Highly conserved and expected to interact with feline, canine, porcine, bovine, and equine ANP.
  • Target Host Species Rat
  • Species Reactivity Bovine (Predicted), Cat (Predicted), Dog (Predicted), Horse (Predicted), Human, Mouse, Pig (Predicted), Rat
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Antibody Isotype IgG
  • Conjugate Unconjugated
  • Immunogen Description Synthetic rat Atrial natriuretic peptide (123-150 aa) conjugated to BSA.
  • Sequence SLRRSSCFGGRIDRIGAQSGLGCNSFRY
  • Purity Description Affinity-purified
  • Format Lyophilized with BSA
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 0.05 mL of sterile-filtered PBS (pH 7.4). Centrifuge to remove any insoluble material.
  • Storage Instructions At least 12 months after purchase at 2 - 8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for up to 6 months for a higher stability and at 2-8°C with an appropriate antibacterial agent for up to 2 weeks.Avoid freeze-thaw cycles
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names Atrial natriuretic factor; ANF; Prepronatriodilatin;
  • Uniprot Number P01161
  • Uniprot Number/Name P01161 (ANF_RAT)
  • Scientific Background The hormone Atrial natriuretic peptide (ANP) is produced by cardiomyocites and is secreted into the bloodstream. ANP acts on convoluted tubules in kidney reducing sodium absorption which results in reducing the blood pressure.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Immunohistochemical staining in convoluted tubules in rat kidney. 4% paraformaldehyde fixed rat kidney crystostat sections (10 µm) were incubated overnight at 4°C with Rabbit polyclonal antibody to rat Atrial Natriuretic Peptide (10 µg/mL) followed by incubation with donkey anti-rabbit Rhodamine Red conjugated secondary antibody (1:200).

    Citations & References