Application DetailsA dilution of 5-10 µg/mL is recommended for immunohistochemistry using formalin fixed and paraffin embedded tissues and for 4% paraformaldehyde fixed frozen tissues. A dilution of 5-15 µg/mL is recommended for immunofluorescence. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetAtrial Natriuretic Peptide
SpecificityHuman; mouse; rat. Highly conserved and expected to interact with feline, canine, porcine, bovine, and equine ANP.
Target Host SpeciesRat
Species ReactivityBovine (Predicted), Cat (Predicted), Dog (Predicted), Horse (Predicted), Human, Mouse, Pig (Predicted), Rat
Immunogen DescriptionSynthetic rat Atrial natriuretic peptide (123-150 aa) conjugated to BSA.
FormatLyophilized with BSA
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 0.05 mL of sterile-filtered PBS (pH 7.4). Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2 - 8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for up to 6 months for a higher stability and at 2-8°C with an appropriate antibacterial agent for up to 2 weeks.Avoid freeze-thaw cycles
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesAtrial natriuretic factor; ANF; Prepronatriodilatin;
Scientific BackgroundThe hormone Atrial natriuretic peptide (ANP) is produced by cardiomyocites and is secreted into the bloodstream. ANP acts on convoluted tubules in kidney reducing sodium absorption which results in reducing the blood pressure.
Immunohistochemical staining in convoluted tubules in rat kidney. 4% paraformaldehyde fixed rat kidney crystostat sections (10 µm) were incubated overnight at 4°C with Rabbit polyclonal antibody to rat Atrial Natriuretic Peptide (10 µg/mL) followed by incubation with donkey anti-rabbit Rhodamine Red conjugated secondary antibody (1:200).