Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH), Rabbit Polyclonal Antibody

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Catalog Number
R-1701

    Product Info

  • Product Name Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH), Rabbit Polyclonal Antibody
  • Product Description google Rabbit anti-Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
  • Alternative Names Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; GAPD; G3PDH; GPDH
  • Application(s) ICC, WB
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Specificity Specific for the ~36-38 kDa Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) protein.
  • Species Reactivity Human, Mouse, Other Mammals (Predicted)
  • Immunogen Description Full length recombinant human GAPDH expressed in and purified from E. coli
  • Conjugate Unconjugated
  • Purity Description Whole serum
  • Regulatory Status For research use only.

    Specifications

  • Product Description Rabbit anti-Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
  • Application(s) ICC, WB
  • Application Details Western Blotting (WB) and Immunocytochemistry (ICC). A dilution of 1:10,000-1:30,000 is recommended for WB. A dilution of 1:1,000-1:10,000 is recommended for ICC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH)
  • Specificity Specific for the ~36-38 kDa Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) protein.
  • Target Host Species Human
  • Species Reactivity Human, Mouse, Other Mammals (Predicted)
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Antibody Isotype IgG
  • Conjugate Unconjugated
  • Immunogen Description Full length recombinant human GAPDH expressed in and purified from E. coli
  • Purity Description Whole serum
  • Format Lyophilized with sodium azide.
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
  • Storage Instructions After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; GAPD; G3PDH; GPDH
  • Uniprot Number P04406
  • Uniprot Number/Name P04406 (G3P_HUMAN)
  • Scientific Background Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. GAPDH may have other roles in the activation of transcription and in the regulation of apoptosis as well as Alzheimer's disease and Huntington's disease. The immunogen used to raise this particular antibody was extensively purified pig GAPDH. This antibody can be used as a loading control for western blotting experiments, allowing comparison between the level of this protein and others in a cell or tissue.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • HeLa cell extract blotted with rabbit anti-GAPDH. Note the single sharp band corresponding to GAPDH.

  • HeLa cells stained with rabbit anti-GAPDH (red), monoclonal to TAF15 (green) and DNA (blue). The antibody reveals strong cytoplasmic staining for GAPDH.


    Left: HeLa cells stained with rabbit antibody to GAPDH (red, 1:2,000) by Immunocytochemistry. Blue: Hoechst nuclear stain. The GAPDH antibody produces diffuse cytoplasmic staining. Right: Western blot analysis of cell cytosolic- or nuclear-enriched fractions, using rabbit antibody to GAPDH (red, 1:20,000, lanes 2-5). [1] protein standard, [2] NIH-3T3 cytosolic, [3] NIH-3T3 nuclear, [4] HeLa cytosolic, and [5] HeLa nuclear fractions. A strong band at 37 kDa corresponds to GAPDH protein, mainly detected in the cytosolic fractions. The same blot was simultaneously probed with mouse antibody to the nuclear RNA binding protein SF3B4 (M-1576-100, green, 1:1,000). In contrast to GAPDH, SF3B4 is exclusively expressed in the nuclear fraction.

    Citations & References

  • General References Morgenegg G, Winkler GC, Hubscher U, Heizmann CW, Mous J, Kuenzle CC. Glyceraldehyde-3-phosphate dehydrogenase is a nonhistone protein and a possible activator of transcription in neurons. J Neurochem. 47:54-62 1986
    Schulze H, Schuler A, Stuber D, Dobeli H, Langern H & Huber G. Rat brain glyceraldehyde-3-phosphate dehydrogenase interacts with the recombinant cytoplasmic domain of Alzheimer's beta-amyloid precursor protein. J Neurochem. 60:1915-22 1993
    Burke JR, Enghild JJ, Martin ME, Jou Y-S, Myers RM, Roses AD, Vance JM & Strittmatter WJ. Huntingtin and DRPLA proteins selectively interact with the enzyme GAPDH. Nature Med. 2: 347-350, 1996.
    Dastoor Z. & Dreyer, J-L. Potential role of nuclear translocation of glyceraldehyde-3-phosphate dehydrogenase in apoptosis and oxidative stress. J. Cell Sci. 114:1643-1653 200100:429-36 (2007).