HeLa cells stained with rabbit anti-GAPDH (red), monoclonal to TAF15 (green) and DNA (blue). The antibody reveals strong cytoplasmic staining for GAPDH.
Left: HeLa cells stained with rabbit antibody to GAPDH (red, 1:2,000) by Immunocytochemistry. Blue: Hoechst nuclear stain. The GAPDH antibody produces diffuse cytoplasmic staining. Right: Western blot analysis of cell cytosolic- or nuclear-enriched fractions, using rabbit antibody to GAPDH (red, 1:20,000, lanes 2-5).  protein standard,  NIH-3T3 cytosolic,  NIH-3T3 nuclear,  HeLa cytosolic, and  HeLa nuclear fractions. A strong band at 37 kDa corresponds to GAPDH protein, mainly detected in the cytosolic fractions. The same blot was simultaneously probed with mouse antibody to the nuclear RNA binding protein SF3B4 (M-1576-100, green, 1:1,000). In contrast to GAPDH, SF3B4 is exclusively expressed in the nuclear fraction.