Left: Analysis of cortical neuron-glial cell culture from E20 with rabbit antibody to GAP43 (green, 1:2,000) by Immunocytochemistry. Cells were co-stained with a mouse antibody to vimentin (red). Blue: DAPI nuclear stain. The GAP43 antibody labels protein expressed in the axonal membrane of neuronal cells, while the vimentin antibody stains intermediate filaments in fibroblasts and other non-neuronal cells. Right: Western blot analysis of tissue and cell lysates using rabbit antibody to GAP43 (green, 1:20,000).  protein standard,  rat brain,  rat spinal cord,  mouse brain,  mouse spinal cord,  SH-SY5Y cells,  C6. The single band at 43 kDa corresponds to GAP43 protein. The GAP43 protein is detected only in the lysates of neuronal origin. Rat glioblastoma C6 cells do not express GAP43 protein (lane 7).