Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. Human Rho-associated protein kinase 2 has a predicted length of 1388 amino acids and MW of 160 kDa. A concentration of 0.5-1.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetRho-associated protein kinase 2
SpecificityHuman (WB, IHC); rat (WB); predicted to react with mouse due to sequence homology;
Target Host SpeciesHuman
Species ReactivityHuman, Mouse (Predicted), Rat
Immunogen DescriptionA synthetic peptide corresponding to a region (35-51) from human Rho-associated protein kinase 2.
Purity DescriptionAffinity purified on antigen column
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesEC 184.108.40.206; Rho-associated; coiled-coil-containing protein kinase 2; p164 ROCK-2; Rho kinase 2; ROCK2; KIAA0619;
Scientific BackgroundRho-associated protein kinase 2 (ROCK2) is a serine/threonine kinase that phosphorylates many important signalling proteins involved in the regulation of cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions complexes as well as malignant cell transformation, tumor invasion and metastasis.