Product DescriptionRabbit anti-BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 (BNIP3) Polyclonal Antibody (Unconjugated), suitable for WB.
Application DetailsWestern Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. Human BNIP3 has a predicted length of 194 residues and MW of 22 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetBCL2/adenovirus E1B 19 kDa protein-interacting protein 3 (BNIP3)
SpecificityThe specificity of this antibody has been confirmed by WB. Human; predicted to react in rat and mouse;
Target Host SpeciesHuman
Species ReactivityHuman, Mouse (Predicted), Rat (Predicted)
Immunogen DescriptionA synthetic peptide corresponding to a region (38-53 aa) from human BNIP3. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
Purity DescriptionAffinity purified on antigen column
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesBCL2/adenovirus E1B 19 kDa protein-interacting protein 3; BNIP3; NIP3;
Scientific BackgroundBCL2/adenovirus E1B 19 kDa protein-interacting protein 3 (BNIP3) is a member of the NIP3 family of proteins. As a homodimer, BNIP3 can overcome BCL2 suppression and induce apoptosis.