Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.5-1.0 µg/mL is recommended for WB. Human Caspase 2 (Isoform ICH-1L) has a predicted length of 452 amino acids and a MW of 51 kDa. A concentration of 1.0-2.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; rat; mouse;
Target Host SpeciesHuman
Species ReactivityHuman, Mouse, Rat
Immunogen DescriptionA synthetic peptide (REGYAPGTEFHRCKEMSEYCSTL) corresponding to a region (417-439) from human Caspase 2
Purity DescriptionAffinity purified on antigen column
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesCASP-2; ICH-1 protease; CASP2; ICH1; NEDD2;
Scientific BackgroundCaspase 2 is a member of the cysteine-aspartic acid protease (caspase) family and acts as both a positive and negative regulator of apoptosis. Overexpression of the full length Caspase 2 (Isoform ICH-1L) in some cell types results in apoptosis whereas the overexpression of an alternative Caspase 2 (ICH-1S) splice variant suppresses apoptosis.
Immunohistochemical staining of paraffin-embedded human mammary cancer sections using Caspase 2 antibody (R-1026-100) at a concentration of 1.0-2.0 µg/ml. Secondary detection with Biotinylated goat anti-rabbit IgG (10 µg/ml).