Application DetailsWestern blotting (0.1-0.5 µg/mL) and direct ELISA (0.1-0.5 µg/mL). Other applications have not been tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetIntegrin alpha-6 (CD49f)
SpecificityRabbit IgG polyclonal antibody for Integrin alpha 6/ITGA6 detection. Tested in WB, and direct ELISA against Human, Mouse, and Rat extracts.
Target Host SpeciesHuman
Species ReactivityHuman, Mouse, Rat
Immunogen DescriptionE.coli-derived human Integrin alpha 6/ITGA6 (CD49f) recombinant protein (amino acids Q189-K917).
Purity DescriptionAffinity Purified
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 uL sterile-filtered, ultrapure water to obtain an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsStore lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks). Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesIntegrin alpha-6; CD49 antigen-like family member F; VLA-6; CD_antigen: CD49f
Scientific BackgroundIntegrin alpha-6/beta-1 (ITGA6:ITGB1) is a receptor for laminin on platelets (By similarity). Integrin alpha-6/beta-1 (ITGA6:ITGB1) is present in oocytes and is involved in sperm-egg fusion (By similarity). Integrin alpha-6/beta-4 (ITGA6:ITGB4) is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome (By similarity). ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:22351760). ITGA6:ITGB4 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed:28873464). Source: uniprot.org. In new work published by L.Barbar [PPR83158] the authors demonstrate that CD49f is an effective marker effective marker for sorting functional human astrocytes from mixed cultures.
Western blot analysis of ITGA6/CD49f expression in cell lysates (1-3) and tissue homogenates (4-6). Lane 1: human placenta tissue lysates, lane 2: human Hela whole cell lysates, lane 3: human Caco-2 whole cell lysates, lane 4: rat kidney tissue lysates, lane 5: rat lung tissue lysates, lane 6: mouse kidney tissue lysates. Loading amount: 50 ug protein per lane. SDS-PAGE: reducing conditions.