Application DetailsWestern Blotting (WB). A concentration of 1.0 µg/mL is recommended for WB. Human IL-1 beta (precursor) has a predicted length of 269 amino acids and MW of 31 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetInterleukin-1 beta (IL-1 beta)
SpecificityThe specificity of this antibody has been confirmed by WB against the antigen. Human;
Target Host SpeciesHuman
Immunogen DescriptionA synthetic peptide corresponding to a region (249-269 aa) from human Interleukin-1 beta.
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesInterleukin-1 beta; IL-1 beta; Catabolin; IL1B; IL1F2;
Scientific BackgroundInterleukin-1 beta (IL-1 beta) belongs to the IL-1 family. IL-1 is produced by activated macrophages and stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity (Ref: SWISSPROT).