Macrophage migration inhibitory factor (MIF), Rabbit Polyclonal Antibody

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Catalog Number
R-1072

    Product Info

  • Product Name Macrophage migration inhibitory factor (MIF), Rabbit Polyclonal Antibody
  • Product Description google Rabbit anti-Macrophage migration inhibitory factor (MIF) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
  • Alternative Names MIF; EC 5.3.2.1; Phenylpyruvate tautomerase; Glycosylation-inhibiting factor; GIF; GLIF; MMIF;
  • Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; rat; predicted to react with mouse due to sequence homology
  • Species Reactivity Human, Mouse (Predicted), Rat
  • Immunogen Description A synthetic peptide (NYYDMNAANVGWNNSTFA) corresponding to a region (98-115) from the C-terminus of human macrophage migration inhibitory factor (MIF).
  • Conjugate Unconjugated
  • Purity Description Affinity purified on antigen column
  • Regulatory Status For research use only.

    Specifications

  • Product Description Rabbit anti-Macrophage migration inhibitory factor (MIF) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
  • Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
  • Application Details Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. Human MIF has a predicted length of 115 amino acids and MW of 12 kDa. A concentration of 0.5-1 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Macrophage migration inhibitory factor (MIF)
  • Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; rat; predicted to react with mouse due to sequence homology
  • Target Host Species Human
  • Species Reactivity Human, Mouse (Predicted), Rat
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Antibody Isotype IgG
  • Conjugate Unconjugated
  • Immunogen Description A synthetic peptide (NYYDMNAANVGWNNSTFA) corresponding to a region (98-115) from the C-terminus of human macrophage migration inhibitory factor (MIF).
  • Purity Description Affinity purified on antigen column
  • Format Lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
  • Storage Instructions At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names MIF; EC 5.3.2.1; Phenylpyruvate tautomerase; Glycosylation-inhibiting factor; GIF; GLIF; MMIF;
  • Uniprot Number P14174
  • Uniprot Number/Name P14174 (MIF_HUMAN)
  • Scientific Background Macrophage migration inhibitory factor (MIF) is a pro-inflammatory cytokine. MIF has a role in the regulation of macrophage function in host defense by counteracting the anti-inflammatory effects of glucocorticoids. MIF is associated with the innate immune response to bacterial pathogens.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

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