Product DescriptionRabbit anti-Mitogen-activated protein kinase 3 (MAPK 3) Polyclonal Antibody (Unconjugated), suitable for WB.
Application DetailsWestern Blotting (WB). A concentration of 1.0 µg/mL is recommended for WB. Human MAPK3 (precursor) has a predicted length of 379 amino acids and MW of 43 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetMitogen-activated protein kinase 3 (MAPK 3)
SpecificityThe specificity of this antibody has been confirmed by WB against the antigen. Human; rat; predicted to react with mouse due to sequence homology;
Target Host SpeciesHuman
Species ReactivityHuman, Mouse (Predicted), Rat
Immunogen DescriptionA synthetic peptide corresponding to a region (365-379 aa) from human Mitogen-activated protein kinase 3 (MAPK3).
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundMitogen activated protein kinase (MAPK), also known as Extracellular signal regulated kinase (ERK), is a family with two homologous members; ERK1 (MAPK3) and ERK2 (MAPK1). MAPK3 is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1 (Ref: SWISSPROT).