SpecificityThe specificity of this antibody has been confirmed by WB against the antigen. Human; mouse; rat;
Species ReactivityHuman, Mouse, Rat
Immunogen DescriptionA synthetic peptide (EARFAGHNFRNPSVL) corresponding to a region (420-434) from the C-terminus of human Neuron-specific enolase (NSE). To enhance the immunological response, this peptide was coupled to carrier protein BSA.
ConjugateUnconjugated
Purity DescriptionAffinity purified on antigen column
Application DetailsWestern Blotting (WB). A concentration of 1.0-2.0 µg/mL is recommended for WB. Human NSE has a predicted length of 434 residues and MW of 47 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetNeuron-specific enolase (NSE)
SpecificityThe specificity of this antibody has been confirmed by WB against the antigen. Human; mouse; rat;
Target Host SpeciesHuman
Species ReactivityHuman, Mouse, Rat
Antibody HostRabbit
Antibody TypePolyclonal
Antibody IsotypeIgG
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide (EARFAGHNFRNPSVL) corresponding to a region (420-434) from the C-terminus of human Neuron-specific enolase (NSE). To enhance the immunological response, this peptide was coupled to carrier protein BSA.
Purity DescriptionAffinity purified on antigen column
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundEnolase is a metalloenzyme that catayzes the reaction between 2-phospho-D-glycerate and phosphoenolpyruvate during glycolysis. Mammalian enolase is composed of 3 subunits; alpha, beta and gamma (Neuron-specific enolase). These subunits can form homodimers or heterodimers. The alpha/gamma heterodimer and the gamma/gamma homodimer are found primarily in neurons.