Application DetailsA dilution of 5-10 µg/mL is recommended for immunohistochemistry using formalin fixed and paraffin embedded tissues and for 4% paraformaldehyde fixed frozen tissues. A dilution of 5-15 µg/mL is recommended for immunofluorescence. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
SpecificityHuman; mouse; rat. Highly conserved and expected to interact with bovine, feline and monkey
Target Host SpeciesHuman
Species ReactivityBovine (Predicted), Cat (Predicted), Human, Mouse, Primate (Predicted), Rat
Immunogen DescriptionSynthetic human Neurotensin peptide (151-163 aa) conjugated to BSA
FormatLyophilized with BSA
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 0.05 mL of sterile-filtered PBS (pH 7.4). Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability and at 2-8°C with an appropriate antibacterial agent. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesNeuromedin N; NTS; NN; NmN; NT; NmN-125;
Immunohistochemical staining in rat brainstem medulla. 4% paraformaldehyde fixed rat brain crystostat sections (10 µm) were incubated overnight at 4°C with Rabbit polyclonal antibody to human Neurotensin (10 µg/mL) followed by incubation with donkey anti-rabbit Rhodamine Red conjugated secondary antibody (1:200). Cell nuclei were counterstained with DAPI.