Application DetailsWestern Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. Human acetylcholine receptor subunit alpha-1 (isoform 2) has a predicted length of 482 amino acids and MW of 55 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesAcetylcholine receptor subunit alpha; CHRNA1; ACHRA; CHNRA;
Scientific BackgroundUpon binding of acetylcholine, the acetylcholine receptor responds by undergoing an extensive change of conformation which affects its alpha and beta subunits and leads to the opening of an ion-conducting channel across the plasma membrane. At least 2 isoforms of the alpha-1 subunit are produced from alternate splicing. Isoform 1 is only expressed in skeletal muscle whereas isoform 2 is constitutively expressed in skeletal muscle, brain, heart, kidney, liver, lung and thymus.