Application DetailsWestern Blotting (WB) and Immunohistochemistry (IHC). A concentration of 1.0 µg/mL is recommended for WB. Human NOS (nNOS-1) has a predicted length of 1,434 residues and MW of 161 kDa. A concentration of 2.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetNitric Oxide Synthase 1 (NOS type 1)
SpecificityThe specificity of this antibody has been confirmed by WB in human and rat and by IHC in rat. Human; rat;
Target Host SpeciesHuman
Species ReactivityHuman, Rat
Immunogen DescriptionA synthetic peptide (IAFIEESKKDTDEVFSS) corresponding to a sequence (1418-1434 aa) from human Nitric Oxide Synthase 1.
Purity DescriptionAffinity purified on antigen column
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative Namesneuronal NOS; nNOS; brain NOS; bNOS; NOS type 1; NOS1; NC-NOS; Constitutive NOS;
Scientific BackgroundNitric oxide synthase (NOS) catalyses the formation of nitric oxide, an important messenger molecule in functions such as homeostasis and synaptic plasticity. NOS is classified into three types: (1) neuronal NOS (nNOS) or brain NOS (bNOS) (2) inducible NOS (iNOS) or macrophage NOS (mNOS) and (3) endothelial NOS (eNOS).
Western Blot using Rabbit polyclonal antibody to human Nitric Oxide Synthase 1 (R-1323-100) at 1 µg/mL:
Lane 1 and 2: Rat brain tissue lysate Lane 3: Rat medulla oblongata tissue lysate Lane 4: MCF-7 Whole cell lysate Lane 5: SMMC Whole cell lysate Lane 6: SW620 Whole cell lysate Lane 7: Colo320 Whole cell lysate