Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. The predicted length of human Smad2 is 467 amino acids and MW of 52 kDa. The predicted length of Smad3 is 425 amino acids and MW of 48 kDa. A concentration of 0.5-1.0 µg/mL is recommended to detect Smad 2/3 in formalin fixed and paraffin embedded tissues as well as formalin/acetone fixed tissues. Heat mediated antigen retrieval is required. A concentration of 0.4-1.0 µg/mL is recommended for IHC-F. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; expected to react with rat and mouse due to sequence homology
Target Host SpeciesHuman
Species ReactivityHuman, Mouse (Predicted), Rat (Predicted)
Immunogen DescriptionA synthetic peptide (EQNGQEEKWCEKAVKS) corresponding to a region from the N-terminus of human Smad2 and Smad3.
Purity DescriptionAffinity purified on antigen column
Scientific BackgroundSmad proteins are essential mediators of signal transduction by the TGF-beta superfamily from the cell surface into the nucleus. Once in the nucleus, Smad proteins target a number of DNA binding proteins to stimulate transcription. Smad4, also known as the common-mediator Smad (co-Smad), interacts with the receptor-regulated Smad proteins (R-SMAD). The R-SMAD class includes Smad2 and Smad3.