Application DetailsA dilution of 5-10 µg/mL is recommended for immunohistochemistry using formalin fixed and paraffin embedded tissues and for 4% paraformaldehyde fixed frozen tissues. A dilution of 5-15 µg/mL is recommended for immunofluorescence. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
SpecificityHuman Somatostatin. Cross reactivity is expected with mouse, rat, other species not yet tested.
Target Host SpeciesHuman
Species ReactivityHuman, Mouse, Rat
Immunogen DescriptionSynthetic Somatostatin peptide (103-116) conjugated to BSA.
FormatLyophilized with BSA
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 0.05 mL of sterile-filtered PBS (pH 7.4). Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for up to six months for a higher stability and at 2-8°C for one week with an appropriate antibacterial agent. Avoid freeze-thaw cycles
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Immunohistochemical staining in rat brainstem medulla. 4% paraformaldehyde fixed rat brain crystostat sections (10 µm) were incubated overnight at 4°C with Rabbit polyclonal antibody to human Somatostatin (10 µg/mL) followed by incubation with donkey anti-rabbit Rhodamine Red conjugated secondary antibody (1:200). Cell nuclei were counterstained with DAPI.