Application DetailsWestern Blotting (WB) and Immunohistochemistry (IHC). A concentration of 1.0 µg/mL is recommended for WB. Human Somatostatin (precursor) has a predicted length of 116 amino acids and MW of 13 kDa. A concentration of 1.0 µg/mL is recommended for IHC to detect the protein in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required. A concentration of 1.0 µg/mL is also recommended for IHC in frozen tissue. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human (WB, IHC); rat (IHC); mouse (IHC);
Target Host SpeciesHuman
Species ReactivityHuman, Mouse, Rat
Immunogen DescriptionA synthetic peptide corresponding to a region (1-14 aa) from the N-terminus of human Somatostatin.
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesGrowth hormone release-inhibiting factor; Somatostatin-282; Somatostatin-14; SST;
Scientific BackgroundThe active forms of Somatostatin are produced by alternate cleavage of the single preproprotein encoded by the Somatostatin gene. This protein is ubiquitously expressed and inhibits the release of many secondary hormones by binding to high-affinity G-protein-coupled Somatostatin receptors.