Application DetailsWestern Blotting (WB) and Immunohistochemistry (IHC). A concentration of 0.1-0.5 µg/mL is recommended for WB. Human UPAR (isoform 1 precursor) has a predicted length of 335 amino acids and MW of 37 kDa. A concentration of 0.5-2.0 µg/mL is recommended for IHC to detect the protein in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesMonocyte activation antigen Mo3; CD87; U-PAR; PLAUR; uPA Receptor;
Scientific BackgroundThis receptor for Urokinase Plasminogen Activator has a role in localizing and promoting plasmin formation. At least 3 isoforms are produced by alternative splicing (Ref: SWISSPROT).
Immunohistochemical staining with Rabbit polyclonal antibody to human Urokinase plasminogen activator surface receptor (R-1617-100) at 2 µg/mL in Human Mammary Cancer Tissue. Secondary biotin conjugated goat anti-rabbit IgG antibody at 10 µg/mL.