Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. Human Vascular endothelial growth factor (isoform VEGF206) has a predicted length of 232 residues and MW of 27 kDa. A concentration of 0.5-1 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Heat antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetVascular endothelial growth factor (VEGF)
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; rat; expected to react with mouse due to sequence homology
Target Host SpeciesHuman
Species ReactivityHuman, Mouse (Predicted), Rat
Immunogen DescriptionA synthetic peptide corresponding to a sequence at the N-terminus of human VEGFA(33-48aa VDIFQEYPDEIEYIFK), identical to the related mouse and rat sequences.
Purity DescriptionAffinity purified on antigen column
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesVEGF-A; Vascular permeability factor; VPF; VEGFA; VEGF;
Scientific BackgroundVascular endothelial growth factor (VEGF) is a disulfide linked homodimeric protein associated with angiogenesis, vasculogenesis and endothelial cell growth. At least 10 isoforms of VEGF are produced by alternative splicing with varying levels of expression. The isoforms VEGF189, VEGF165 and VEGF121 are widely expressed whereas the isoforms VEGF206 and VEGF145 are uncommon. Isoforms VEGF165 and VEGF121 are freely secreted whereas isoform VEGF189 remains cell associated.