Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. Human ICAM1 (precursor) has a predicted length of 532 residues and a MW of 58 kDa. A concentration of 0.5-1 µg/mL is recommended to detect ICAM1 in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetIntercellular adhesion molecule 1 (ICAM1)
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human
Target Host SpeciesHuman
Immunogen DescriptionA synthetic peptide (YNRQRKIKKYRLQQ) corresponding to a region (503-516) from the C-terminus of human ICAM1.
Purity DescriptionAffinity purified on antigen column
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesICAM1; ICAM-1; Intercellular adhesion molecule 1; CD54;
Scientific BackgroundIntercellular adhesion molecule 1 (ICAM1) is a single-pass type 1 membrane glycoprotein that belongs to the ICAM family. ICAM1 is typically expressed on endothelial cells and cells of the immune system. ICAM-1 is also expressed in all types of glial cells including astrocytes and microglia.
Immunohistochemical staining of paraffin-embedded human mammary cancer sections using ICAM1 antibody (R-1025-100) at a concentration of 1.0-2.0 µg/ml. Secondary detection with Biotinylated goat anti-rabbit IgG (10 µg/ml).
General ReferencesSatoh J. et al. Cytokine-induced expression of intercellular adhesion molecule-1 (ICAM-1) in cultured human oligodendrocytes and astrocytes. J Neuropathol Exp Neurol. 1991 May;50(3):215-26. Verbeek M.M. Accumulation of intercellular adhesion molecule-1 in senile plaques in brain tissue of patients with Alzheimer's disease. Am J Pathol. 1994 January; 144(1): 104-11 Heppner F.L. et al. Vitamin E induces ramification and downregulation of adhesion molecules in cultured microglial cells. Glia. 1998 Feb;22(2):180-8. Yang Y. et al. Evaled expression of ICAM-1 and its ligands in the rat spinal cord following lipopolysaccharide intraspinal injection. Neuromolecular Med. 2008;10(4):385-9