Left: Detection of alpha-internexin expression (green) in rat cerebellum by Immunohistochemistry. The rabbit antibody to alpha-internexin was used at 1:2,000 dilution, and section was co-stained with chicken antibody to GFAP (C-1373-50, red, 1:5,000). Blue: DAPI nuclear stain. IHC Method: Following transcardial perfusion with 4% paraformaldehyde, brain was post-fixed for 24 hours, cut to 45 um, and free-floating sections were stained. The alpha-internexin antibody selectively stains axons and dendrites of neuronal cells, in particular Purkinje cells and parallel fibers the axons of granule cells. The GFAP antibody labels network of glial cells, such as astrocytes in the granule cell layer and white matter and Bergmann glia in the molecular layer. Right: Western blot analysis of whole tissue lysates using rabbit antibody to alpha-internexin (green, 1:10,000).  protein standard,  mouse spinal cord,  rat spinal cord,  bovine spinal cord. Major bands in the 64-66 kDa range correspond to alpha-internexin. The alpha-internexin protein from different species is known to vary slightly in molecular weight as determined by SDS-PAGE.