Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. Rat NMDAR1 (isoform A) has a predicted length of 938 residues and molecular weight of 106 kDa. A concentration of 0.5-1 µg/mL is recommended to detect NMDAR1 in paraffin embedded tissues. Heat mediated antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetN-methyl-D-aspartate receptor 1 (NMD-R1)
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; Rat; predicted to react with mouse due to sequence homology;
Target Host SpeciesHuman
Species ReactivityHuman, Mouse (Predicted), Rat
Immunogen DescriptionA synthetic peptide corresponding to a sequence at the N-terminus of human NMDAR1 (36-53aa RKHEQMFREAVNQANKRH), identical to the related rat and mouse sequences.
Purity DescriptionAffinity purified on antigen column
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at -20°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundN-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate receptors. NMDA receptor channels are formed from heteromers composed of three different subunits: the key receptor zeta subunit NMDAR1 (GRIN1) with an NMDAR2 epsilon subunit: NMDAR2A (GRIN2A), NMDAR2B (GRIN2B), NMDAR2C (GRIN2C), or NMDAR2D (GRIN2D) and a third subunit: GRIN3A or GRIN3B. At least 7 isoforms of NMDAR1 are produced by alternative splicing.
Western blotting analysis of NMDAR1 expression in rat tissue and cell lines. From left to right: rat brain tissue lysate; rat brain tissue lysate; rat liver tissue lysate; MM231 cell lysate; HELA cell lysate