Product DescriptionRabbit anti-PRKR-like endoplasmic reticulum kinase (phosphorylated and non phosphorylated) (PERK) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Application DetailsWestern Blotting (WB) and Immunoprecipitation (IP). A dilution of 1:500 is recommended for WB. A dilution of 30 µL of antibody in a total reaction mixture of 500 µL is recommended for IP. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetPRKR-like endoplasmic reticulum kinase (phosphorylated and non phosphorylated) (PERK)
SpecificityThis antiserum is known to recognise both phosphorylated and non phosphorylated mouse PERK.
Target Host SpeciesMouse
Immunogen DescriptionA recombinant peptide from mouse PERK.
Purity DescriptionWhole serum
FormatLiquid. 50% glycerol
Storage InstructionsAliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Western Blot using mouse cultured astrocyte extracts at rest (Cntrl) and after 60 mins of ER stress induction by oxygen glucose deprivation (OGD). The left panel membrane was probed with Rabbit polyclonal antibody to mouse PERK: Whole serum (R-1333-100). The right membrane was similarly probed in combination with the antigenic peptide used to generate the antibody. Image taken from Bollo et al (2010) Figure S4.