Left: Analysis of adult mouse cerebellum with rabbit antibody to FOX3/NeuN (red, 1:5,000) by Immunohistochemistry. Section was co-stained with chicken antibody to GFAP (C-1373-50, green, 1:5,000). IHC Method: Following transcardial perfusion with 4% paraformaldehyde, brain was post-fixed for 24 hours and 45 µm free-floating sections were stained. The FOX3/NeuN antibody stains the nuclei of neurons in the cerebellar granule layer. The GFAP antibody stains the processes of Bergmann glia in the molecular layer and astroglia in the granule and white matter layers. Right: Western blot analysis of whole brain lysates using rabbit antibody to Fox3/NeuN (green, 1:1,000).  protein standard,  mouse brain,  rat brain. Bands at 46-48 kDa correspond to protein isotypes of the Fox3/NeuN protein.