Application DetailsIHC, WB, ELISA. This antibody works in IHC on frozen or wax embedded tissues. Antigen retrieval has been used in testing but may not be necessary. Typical working dilutions for light microscopy are 1:500 to 1:1,000 and IF of 1:50 to 1:100. For WB a dilution range of 1: 1,000 to 1: 4,000 is recommended. A dilution of 1: 1,000 to 1: 4,000 is recommended for ELISA. This antibody stains non-ciliated bronchiolar cells in rat airways and specific kidney tubule cells. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
SpecificityHuman and rat Peroxiredoxin 1 Human; rat;
Target Host SpeciesHuman
Species ReactivityHuman, Rat
Immunogen DescriptionA synthetic peptide (PLVSDPKRTIAQDY) corresponding to the region 103-116 amino acids of human Peroxiredoxin-1 conjugated to diptheria toxin.
Purity DescriptionWhole serum
FormatLyophilized serum with 0.02% thimerosal
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Immunohistochemical staining of non-ciliated bronchiolar cells in rat airways. Incubated with Rabbit polyclonal antibody to Peroxiredoxin-1 (R-1590-100) at 1:100. Secondary antibody Jackson donkey anti-rabbit conjugated to Cy3 at 1:200.