Product DescriptionRabbit anti-Podocin Polyclonal Antibody (Unconjugated), suitable for WB.
Application DetailsWestern Blotting (WB). A concentration of 1.0 µg/mL is recommended for WB. Rat Podocin (isoform 1) has a predicted length of 383 amino acids and MW of 42 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
SpecificityThe specificity of this antibody has been confirmed by WB against the antigen. Rat; predicted to react with mouse and human due to sequence homology;
Target Host SpeciesHuman
Species ReactivityHuman (Predicted), Mouse (Predicted), Rat
Immunogen DescriptionA synthetic peptide (CVKVVQEYERVIIFRLGH) corresponding to a region (124-141 aa) from human Podocin.
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundPodocin belongs to the band 7/mec-2 family and plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton (Ref: SWISSPROT).