Product DescriptionRabbit anti-Protein S100-B Polyclonal Antibody (Unconjugated), suitable for WB.
Application DetailsWestern Blotting (WB) and Immunocytochemistry (ICC). A concentration of 0.1-0.5 µg/mL is recommended for WB. For ICC, a concentration of 0.2-2.0 µg/mL is recommended. Human S100b protein (precursor) has a predicted length of 92 amino acids and MW of 11 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
SpecificityThe specificity of this antibody has been confirmed by WB against the antigen. Human (WB); rat (WB); predicted to react with mouse due to sequence homology;
Target Host SpeciesHuman
Species ReactivityHuman, Mouse (Predicted), Rat
Immunogen DescriptionA synthetic peptide corresponding to a region (64-78 aa) at the C-terminus from human Protein S100 beta (S100b).
Purity DescriptionAffinity purified against peptide immunogen
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsStore at -20°C (lyophilized formulations). After reconstitution, store at 2-8°C short-term (1 month). Aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesS100B; Calcium binding protein B1; S-100 protein beta chain; S-100 protein subunit beta; S100 calcium-binding protein B;
Scientific BackgroundProtein S100 beta belongs to the S100 family of proteins containing 2 EF-hand calcium binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and are involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Among brain cells, S100 beta is often used as a marker for astrocytes. The 2 subunits of S100 protein are the products of separate genes. The beta subunit is present in all S100 positive cells and tumours whereas the alpha subunit is detectable only in specific tissues such as heart, skeletal muscle and brain. The S100 protein is a homodimer composed of two alpha subunits (S100a) or two beta subunits (S100b) or as a heterodimer with one alpha and beta subunit (S100ab or S100ao).
Western blot analysis of S100-B expression in tissue and cell lysates.
Detection of protein S100 beta (green) in rat DRG neurons and Schwann cells in E14 mixed cultures by Immunocytochemistry, using rabbit antibody to S100 beta (R-1734-100). Image courtesy of QBM Cell Science.