SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Rat;
Species ReactivityRat
Immunogen DescriptionA synthetic peptide (TLNKTGHPSCRPILT) corresponding to a region (14-28) from rat Mas-related G-protein coupled receptor member X1. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
ConjugateUnconjugated
Purity DescriptionAffinity purified on antigen column
Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. Rat MRGPRX1 has a predicted length of 323 residues and MW of 37 kDa. A concentration of 0.5-1.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetMas-related G-protein coupled receptor member X1
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Rat;
Target Host SpeciesRat
Species ReactivityRat
Antibody HostRabbit
Antibody TypePolyclonal
Antibody IsotypeIgG
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide (TLNKTGHPSCRPILT) corresponding to a region (14-28) from rat Mas-related G-protein coupled receptor member X1. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
Purity DescriptionAffinity purified on antigen column
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundMas-related G-protein coupled receptor member X1 (MRGPRX1) is an opiod receptor that is uniquely localized in a subset of small dorsal root and trigeminal sensory neurons. MRGPRX1 is thought to be involved in the regulation of sensory neurons.