Application DetailsWestern Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. Human SCN1B has a predicted length of 218 amino acids and MW of 25 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetSodium Channel subunit beta-1
SpecificityThe specificity of this antibody has been confirmed by WB against the antigen. Human; rat; predicted to react with mouse due to sequence homology;
Target Host SpeciesHuman
Species ReactivityHuman, Mouse (Predicted), Rat
Immunogen DescriptionA synthetic peptide (YENEVLQLEEDERFEGRVVWNGS) corresponding to a region (73-95 aa) from human Sodium Channel subunit beta-1 (SCN1B).
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundSCN1B is a single-pass type I membrane protein abundantly expressed in skeletal muscle, heart and brain (Ref: SWISSPROT). The SCN1B forms part of a heterotrimeric complex of voltage-gated sodium channels involved in the generation and propagation of action potentials in muscle and neuronal cells.