Product NameUbiquitin C Terminal Hydrolase 1 (UCHL1), Rabbit Polyclonal Antibody
Product DescriptiongoogleRabbit anti-Ubiquitin C Terminal Hydrolase 1 (UCHL1) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Alternative NamesUbiquitin carboxyl-terminal hydrolase isozyme L1; UCH-L1; Neuron cytoplasmic protein 9.5; PGP 9.5; PGP9.5; Ubiquitin thioesterase L1; UCHL1;
Application(s)ICC, WB
Antibody HostRabbit
Antibody TypePolyclonal
SpecificityThe specificity of this antibody has been confirmed by WB. This antibody detects ~24 kDa UCHL1 enzyme. Suitable control tissue is rat spinal cord or peripheral nerve homogenate. Hu, Rat, Ms, Bov, Por
Species ReactivityBovine, Chicken, Horse, Human, Mouse, Pig, Rat
Immunogen DescriptionRecombinant full length human Ubiquitin C Terminal Hydrolase 1 (UCHL1) purified from E. coli.
Product DescriptionRabbit anti-Ubiquitin C Terminal Hydrolase 1 (UCHL1) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Application(s)ICC, WB
Application DetailsWestern Blotting (WB) and Immunocytochemistry (IC). A dilution of 1:1,000 - 1:2,000 is recommended for WB. A dilution of 1:500-1:1,000 is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetUbiquitin C Terminal Hydrolase 1 (UCHL1)
SpecificityThe specificity of this antibody has been confirmed by WB. This antibody detects ~24 kDa UCHL1 enzyme. Suitable control tissue is rat spinal cord or peripheral nerve homogenate. Hu, Rat, Ms, Bov, Por
Target Host SpeciesHuman
Species ReactivityBovine, Chicken, Horse, Human, Mouse, Pig, Rat
Antibody HostRabbit
Antibody TypePolyclonal
Antibody IsotypeMixed
ConjugateUnconjugated
Immunogen DescriptionRecombinant full length human Ubiquitin C Terminal Hydrolase 1 (UCHL1) purified from E. coli.
Purity DescriptionWhole serum
FormatLyophilized, without preservatives.
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesUbiquitin carboxyl-terminal hydrolase isozyme L1; UCH-L1; Neuron cytoplasmic protein 9.5; PGP 9.5; PGP9.5; Ubiquitin thioesterase L1; UCHL1;
Scientific BackgroundThis enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. The enzyme also binds to free monoubiquitin and may prevent its degradation in lysosomes (ref: SWISSPROT).
Image shows rat mixed neuron/glial cultures stained with Rabbit polyclonal antibody to Ubiquitin C Terminal Hydrolase 1 R-1408-50 (green). Blue is a DNA stain. Note that the Ubiquitin C Terminal Hydrolase 1 (UCHL1) antibody stains neurons strongly and specifically, and that the staining is concentrated in the cell bodies, though some does extend into the dendrites also. Surrounding glial cells are not stained with this antibody, though many are present as visualized using the DNA stain.
Left: Analysis of SH-SY5Y cells for UCHL1 expression with rabbit antibody to UCHL1 (green, 1:1,000) by Immunocytochemistry. Cells were co-stained with mouse antibody to fibrillarin (M-1372-250, red, 1:1,000). Blue: DAPI nuclear stain. The UCHL1 antibody produces strong staining of the cellular cytoplasm of these cells which share many properties with neurons, while the fibrillarin antibody specifically labels nucleoli. Right: Western blot analysis of tissue and cell lysates using rabbit antibody to UCHL1 (red, 1:2,000, lanes 2-7). [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y cells. The single band at 24 kDa corresponds to the UCHL1 protein. UCHL1 is detectable in CNS extracts and cells with neuronal properties, but not in HeLa, NIH-3T3 and other non-neuronal cells. The blot was simultaneously probed with a mouse antibody to HSP60 (green).
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