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Renin-1, Human, Mouse, Rat, ELISA assay
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US$547.00
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Catalog Number
BEK-2351
- Product Name Renin-1, Human, Mouse, Rat, ELISA assay
- Product Description The mouse Renin-1 kit is a sandwich ELISA. The capture antibody is a rat monoclonal mouse Renin-1 antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known mouse Renin-1 concentration are added to these wells and allowed to complex with the bound mouse Renin-1 antibody. A biotinylated mouse Renin-1 goat polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of mouse Renin-1 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse Renin-1 in samples such as culture supernatant, cell lysates, serum, plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names Angiotensinogenase; Kidney renin; REN1; Ren-1;
- Application(s) ELISA
- Specificity Mouse Renin-1 protein
- Species Reactivity Human, Mouse, Rat
- Immunogen Description The standard and immunogen for this kit is recombinant Mouse Renin-1 expressed in a NSO expression system; Immunogen sequence: S72-R402
- Range 62.5 pg/mL - 4,000 pg/mL
- Sensitivity < 10 pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The mouse Renin-1 kit is a sandwich ELISA. The capture antibody is a rat monoclonal mouse Renin-1 antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known mouse Renin-1 concentration are added to these wells and allowed to complex with the bound mouse Renin-1 antibody. A biotinylated mouse Renin-1 goat polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of mouse Renin-1 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse Renin-1 in samples such as culture supernatant, cell lysates, serum, plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Application(s) ELISA
- Application Details ELISA. For the quantification of Renin-1 in Culture Supernatant, Cell Lysates, Serum, Plasma (Heparin), Plasma (EDTA). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Renin-1
- Specificity Mouse Renin-1 protein
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Mouse
- Species Reactivity Human, Mouse, Rat
- Immunogen Description The standard and immunogen for this kit is recombinant Mouse Renin-1 expressed in a NSO expression system; Immunogen sequence: S72-R402
- Range 62.5 pg/mL - 4,000 pg/mL
- Sensitivity < 10 pg/mL
- Sample Type Cell Lysates, Culture Supernatant, Plasma (EDTA), Plasma (Heparin), Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), Mouse Renin-1 protein standards, Mouse Renin-1 biotinylated antibody, Avidin-Biotin-Peroxidase Complex, sample, antibody and avidin-biotin peroxidase diluents, substrate reagent, stop solution, wash buffer, plate sealers and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date 6 months after date of receipt if unopened
- Alternative Names Angiotensinogenase; Kidney renin; REN1; Ren-1;
- Uniprot Number P06281
- Uniprot Number/Name P06281 (RENI1_MOUSE)
- Scientific Background Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
