Product DescriptionRabbit anti-SARS-CoV spike protein Polyclonal Antibody (Unconjugated), suitable for ELISA.
Application DetailsSARS-CoV Spike antibody can be used for the detection of SARS-CoV Spike protein in ELISA. It will detect 2 ng of free peptide at 1 μg/mL.
TargetSARS-CoV spike protein
Specificity Predicted reactivity based on immunogen sequence: SARS-CoV2 Spike protein: (identity 56%, homology 69%)
Target Host SpeciesVirus
Immunogen DescriptionAnti-SARS-CoV Spike antibody (R-1943-100) was raised against a peptide corresponding to 16 amino acids near the center of SARS-CoV Spike glycoprotein.
The immunogen is located within amino acids 410-460 of SARS-CoV Spike.
HomologyPredicted reactivity based on immunogen sequence: SARS-CoV2 Spike protein: (identity 56%, homology 69%)
Isoform InformationSARS-CoV Spike has one isoform (1255aa).
Purity DescriptionAffinity-purified via peptide column.
FormatLiquid. SARS-CoV Spike Antibody is supplied in PBS containing 0.02% sodium azide. Conc.1 mg/mL
Reconstitution InstructionsSpin vial briefly before opening.
Storage InstructionsSARS-CoV Spike antibody can be stored at 2-8°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesSARS-CoV Spike Antibody: E2, Spike glycoprotein, E2, S glycoprotein
Scientific BackgroundSARS-CoV Spike Antibody: A novel coronavirus has recently been identified as the causative agent of SARS (Severe Acute Respiratory Syndrome). Coronaviruses are a major cause of upper respiratory diseases in humans. The genomes of these viruses are positive-stranded RNA approximately 27-31kb in length. SARS infection can be mediated by the binding of the viral spike protein, a glycosylated 139 kDa protein and the major surface antigen of the virus, to the angiotensin-converting enzyme 2 (ACE2) on target cells. This binding can be blocked by a soluble form of ACE2.
Figure 1 Western Blot Validation with Human Recombinant Protein Loading: 50 ng per lane of human SARS-CoV-2 Spike S1 recombinant protein. Antibodies: SARS-CoV-2/SARS-CoV Spike R-1943-100 (4 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Figure 1 ELISA Test
Antibodies: SARS-CoV Spike Antibody, R-1943-100 (1 μg/mL). A direct ELISA was performed using immunogen as coating antigen and the anti-SARS-CoV Spike antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 2 ng/mL to 1000ng/mL.